Effect of obestation and treadmill exercise on lipid profile and serum malondialdehyde (MDA) levels in rat model
Abstract
Objective: The present study investigates the effect of intraperitoneal (IP) administered obestatin, short duration treadmill exercise on serum malondialdehyde (MDA) and lipid profile in the rat model. Materials and Methods: Forty-five rats weighing 100 ± 5g were selected and divided into five experimental groups; Group 1/control was normally maintained and received no solution or exercise included in obestatin. Group 2 (placebo) was treated with basal solution (50 ml of distilled water). Group 3 was referred to as the obestatin group, which underwent injection during the experiment with obestatin solution (10 microgram obestatin/mouse). Group 4 was known as the treadmill group that underwent the exercise protocol (15-day treadmill training) and group 5, known as the obestatin and treadmill (OT) group, underwent the obestatin injection and exercise protocol. . Exercise groups were trained for 3 weeks. Results: the treadmill group showed a decrease in total cholesterol (TC) concentration (67.67 mg/dl), compared to the control group and the obestatin group (79.00 and 78.33 mg/dl, respectively) . As with the CT level, triglyceride and HDL-cholesterol (HDL-C) decreased in the treadmill group (triglyceride: 70.66 mg/dl compared to 87.67 mg/dl in the control and HDL- C: 46.10 mg/dl compared to 57.33 mg/dl in control; p < 0.05). In addition, the MDA level decreased in the treadmill and treadmill-obestatin groups. Conclusion: Short-term treadmill exercise significantly reduced plasma lipid profile and MDA levels in the rat model. However, obestatin administration had no significant effect on lipid profile and MDA. Furthermore, administration of obestatin in combination with treadmill exercise had no synergistic effect on plasma lipids, MDA level and total protein in rats.
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