Determination of digestive enzymes inhibition activity in vegetable protein based supplements available in international commerce
Abstract
Proteins are important components of the human diet and play an essential role as structural and functional elements of living systems. Their nutritional value will depend, among other characteristics, on the amino acid profile, bioavailability, allergenicity, among other factors, and the presence of enzymatic inhibitors may decrease it. Protease inhibitors are traditionally recognized as an anti-nutritional factor and can be easily denatured by thermal processing, although some residual activity may remain in commercially produced products. The present study aimed to determine the specific activity of inhibition for trypsin, chymotrypsin and pepsin in supplements based on isolated plant protein by obtaining the crude extract, determination of soluble proteins and quantification of inhibitory activity through specific tests. All supplements analyzed showed inhibitory activity for trypsin, chymotrypsin and pepsin, and soybean supplements showed the highest inhibitory activity for proteases. In this report, we concluded that the use of these supplements has a negative influence due to the presence of enzymatic inhibitors, reducing their functionality for hypertrophy purposes.
References
-Araújo, J. M. A. Química de Alimentos: Teoria e Prática. Viçosa. UFV. 2011. 601p.
-Barros, J. G. A.; Moraes, R. M. A.; Piovesan, N. D.; Barros, E. G.; Moreira, M. A. Efeito do inibidor de protease Kunitz sobre níveis de lipoxigenases em sementes de soja. Ciência e Agrotecnologia. Vol. 32. Núm. 4. p. 1126-1132. 2008.
-Bonett, L. P.; Baumgartner, M. S. T.; Klein, A. C.;Silva, L. I. Compostos nutricionais e fatores antinutricionais do feijão comum (Phaseolus Vulgaris L.). Arq. Ciênc. Saúde Unipar. Vol. 11. Núm. 3. p. 235-246.2007.
-Boye, J.; Wijesinha-Bettoni, R.I; Burlingame, B.Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method. British Journal of Nutrition. Vol. 108. Num. 2. p. 183-211. 2012.
-Cardoso, L.R.; e colaboradores. Atividade de inibidores de proteases em linhagens de soja geneticamente melhoradas. Alimentos e Nutrição Araraquara. Vol. 18. Num. 1. p. 19-26. 2008.
-Cazetta, J.O.; e colaboradores. Comparação de aspectos químicos e tecnológicos de grãos verdes e maduros de guandu com os de feijão-comum e ervilha. Alimentos e Nutrição Araraquara. Vol. 6. Num. 1. 2009.
-Clemente, A.; e colaboradores. The effect of variation within inhibitory domains on the activity of pea protease inhibitors from the Bowman-Birk class. Protein expression and purification. Vol. 36. Num. 1. p. 106-114. 2004.
-Conceição, L. L.; Duarte, M. S.L.; Pereira, C. A. S.; Souza, E. C. G. Determinação da atividade in vitro de Inibidores de Tripsina em feijão (Phaseolus Vulgaris L.)-preto, albumina e globulina. Alimentos e Nutrição, Araraquara. Vol. 21. Num. 3. p. 373-376. 2010.
-Del-Vechio, G.; Corrêa, A. D.; Abreu, C. M. P.; Santos, C. D.; Cienc. Agrotecnol. Vol. 29. 2005.
-Domoney, C.; e colaboradores. Multiple isoforms of Pisum trypsin inhibitors result from modification of two primary gene products. FEBS letters. Vol. 360. Num. 1. p. 15-20. 1995.
-Egbert, W. R.; Payne, C. T. Plant proteins. In R. Tarte (Ed.), Ingredients in meat products, properties, functionality and applications. Berlin: Springer. p. 111-131. 2009.
-Fang, E. F.; Wong, J. H; Bah, C. S. F.; Lin, P.; Tsao, S. W; Ng, T. B.; Biochem. Biophys. Res. Commun. Vol. 396. Num. 806. 2010.
-Ferri, P. Extração de proteínas de folhas de mandioca (Manihot esculenta Crants) para obtenção de concentrado protéico. 2006. 112f. Dissertação (Mestrado em Engenharia Agrícola),Universidade Estadual do Oeste do Paraná (Unioeste), Cascavel-PR.
-Gilani, G. Sarwar; Xiao, Chao Wu; Cockell, Kevin A. Impact of Antinutritional Factors in Food Proteins on the Digestibility of Protein and the Bioavailability of Amino Acids and on Protein Quality. British Journal of Nutrition. Vol. 108. Num. 2. p. 315-332. 2012. Disponível em: <http://dx.doi.org/10.1017/s0007114512002371>.
-Giri, A.P.; Kachole, M.S. Amylase inhibitors of pigeon pea (Cajanus cajan) seeds. Phytochemistry. Vol. 47. p. 197-202. 2004.
-Gomes, G.S.; e colaboradores. Caracterização do consumo de suplementos nutricionais em praticantes de atividade física em academias. Medicina (Ribeirão Preto. Online). Vol. 41. Num. 3. p. 327-331. 2008.
-Kakade, M. L.; Simons, N.; Liener, I. E. An evaluation of natural vs. synthetic substrates for measuring the antitryptic activity of soybean samples. Cereal Chemistry. Vol. 46. Num. 5. p. 518-526. 1969.
-Latorres, J.M.; e colaboradores. Isolado protéico de farinha de arroz: caracterização e propriedades funcionais. VETOR- Revista de Ciências Exatas e Engenharias. Vol. 24. Num. 1. p. 53-65. 2016.
-Monteiro, M.R.P.; e colaboradores. Qualidade protéica de linhagens de soja com ausência do Inibidorde tripsina kunitze das isoenzimas Lipoxigenases. Revista de Nutrição. Vol. 17. Num. 2. p. 195-205. 2004. Disponível em: <http://dx.doi.org/10.1590/s1415-52732004000200006.X>
-Petrovic, G.; Nikolic, Z.; Dordevic, V.Endonuclease heteroduplex mismatch cleavage for detecting matation genetic variation of trypsin inihibitors in soybean. Institute of Field and Vegetables Crops. Vol. 49. Num. 2.p. 102-108. 2014.
-Pires, C.V.; e colaboradores. Qualidade nutricional e escore químico de aminoácidos de diferentes fontes protéicas. Ciênc. Tecnol. Aliment. Vol. 26. Num. 1. p. 179-187. 2006.
-Proll, J., Petzke, J., Ezeagu, E.I., Metges, C.C. Low nutritional quality of unconventional tropical crop seeds in rats. Journal of Nutrition, Bethesda. Vol. 128. Num. 11. p. 2014-2022. 1998.
-Ribeiro, A. N. C. R.; Dissertação de Mestrado, Universidade Federal do Rio Grande do Norte, Brasil, 2013.
-Richardson, M. Seed storage proteins: the enzyme inhibitors. In: L.J. Rogers. Methods in plant biochemistry. London: Academic Press. Vol. 5. p. 259-305.1991.
-Sack, M.; e colaboradores. The increasing value of plant-made proteins. Current Opinion In Biotechnology. Vol. 32. p. 163-170. 2015. Disponível em: <http://dx.doi.org/10.1016/j.copbio.2014.12.008>.
-Schulpis, K.H.; e colaboradores. L-cysteine supplementation protects the erythrocyte glucose-6-phosphate dehydrogenase activity from reduction induced by forced training. Clin. Biochem. Vol. 39. Num. 10. p. 1002-1006. 2006.
-Seibel, N. F.; Beléia, A. D. P.; Braz. J. Food Technol. Vol. 12. Num. 113. 2009.
-Serquiz, A. C.; Dissertação de Mestrado, Universidade Federal do Rio Grande do Norte, Brasil, 2012.
-Serquiz, A. C.; e colaboradores. Supplementation with a new trypsin inhibitor from peanut is associated with reduced fasting glucose, weight control, and increased plasma CCK secretion in an animal model. Journal of Enzyme Inhibition and Medicinal Chemistry. Vol. 31. Num. 6. p. 1261-1269. 2016.
-Sgarbieri, V.C. Propriedades físico-químicas e nutricionais de proteínas de feijão (Phaseolus vulgaris,l.) var. Rosinha G2. Campinas, 1979. 207p. Tese (Livre-Docência) -Faculdade de Engenharia de Alimentos e Agrícola, Universidade Estadual de Campinas, 1979.
-Singh, A.; Sahi, C.; Grover, A. Chymotrypsin protease inhibitor gene family in rice: Genomic organization and evidence for the presence of a bidirectional promoter shared between two chymotrypsin protease inhibitor genes. Gene. Vol. 428. Num. 1-2. p. 9-19. 2009. Disponível em: http://dx.doi.org/10.1016/j.gene.2008.09.028.
-Tur-Sinai, A.; Birk, Y.; Gertler, A.; Rigbi, M.; Biochim. Biophys. Acta. Vol. 263. Num. 666.1972.
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